Luka Modric is expected to lead Croatia at what could be his final World Cup as Zlatko Dalic's side aim to continue their remarkable record of success on football's biggest stage despite fitness concerns over their captain.
Croatia and AC Milan's Luka Modric sustained the injury following a clash of heads with Juventus midfielder Manuel Locatelli during their Serie A match on Sunday.
Harry Kane struck twice while Jude Bellingham and Marcus Rashford delivered the knockout blows as England survived a back-and-forth battle to beat Croatia 4-2 on Wednesday and launch their World Cup campaign in a heavyweight clash that lived up to its billing.
"What I've seen so far, when you take a look at their selection of players, their quality, skills and value, then it is indeed terrifying."
Croatia have no major injury concerns going into Sunday's World Cup final despite having played three 120-minute matches
The clash marks the fourth time the Croatia and Spain have met at the Euros.
Croatia beat Brazil in the quarter-finals, but for Dalic, Saturday's bronze medal game was the highlight of their tournament.
'I have calmly accepted that, and since I need my players fit and ready to play, I have made this decision'
Croatia achieved their best-ever result in the tournament in losing Sunday's match to France, surpassing the third place finish of 1998.
Asked to rate Croatia's success in the past four years, Dalic said the 2018 semi-final win over England was so far their biggest match.
The Spaniards, looking for a record fourth Euro crown, struck twice in three minutes, with Fabian Ruiz setting up Alvaro Morata in the 29th and firing in himself a little later.
All other players and staff tested negative
Croatia coach Zlatko Dalic admitted his team rode their luck against Belgium but said he was pleased with the way they controlled the match for large stretches.
'But that in no way diminishes France's win. Maybe we were a bit unlucky, in the first six games we were favoured by luck'
Ivan Rakitic an integral part of the Croatia side who reached the World Cup final in Russia before losing 4-2 to France.
How they strategised, motivated and led their teams to victory.
'We had a fantastic goalkeeper today, Livakovic, he was great, he was insurmountable, he defended the penalties in a very strong, stable manner.'
Spain's 'vertical football' too much for ageing Croatia
Modric was unable to dictate play as much as he normally would
Croatia thrives on being considered underdogs and warned that the 2018 World Cup runners-up have what it takes to reach the final again.
Croatia and Morocco will face off in Qatar for a second time after playing out a 0-0 draw in their group opener
Like a medieval army picking an elevated spot to engage in battle, Albania were happy to concede territory up the field in favour of packing the final third, safe in the knowledge that Croatia would not hurt them.
They also topped their Nations League group ahead of World Cup holders France, Denmark and Austria, and head into the tournament on the back of a winning streak that culminated in a 1-0 victory over Saudi Arabia in their final warm-up friendly.
Denmark's Christian Eriksen was given CPR by medical staff on the pitch during the Euro 2020 match against Finland before being rushed to a nearby hospital.
Neymar will have extra motivation against Croatia, as he is one goal away from equalling Pele's record
Cristiano Ronaldo, Luka Modric and Mohamed Salah have been nominated as finalists for The Best FIFA Men's Player Award, world soccer's governing body announced on Monday.
IMAGES from the Euro 2024 Group B match between Croatia and Albania at Hamburg Volksparkstadion, in Hamburg, Germany, on Wednesday.
Gritty Croatia came back from 1-0 in extra-time to claim a 4-2 penalty shootout victory and with it a spot in the semi-finals.
"Mateo, Luka and Marcelo are the best Croatia midfield in history. I don't think it can be repeated. When you pass them the ball it is safer than having your money in the bank," the defender said.
Croatia coach Zlatko Dalic says he will turn to his Barcelona midfielder Ivan Rakitic for ideas on how to stop Argentina's Lionel Messi in Thursday's Group D match between the two teams.
Croatia's elated coach Zlatko Dalic lauded his captain Luka Modric who missed a penalty in extra time but then scored another in a shootout victory over Denmark on Sunday that put them in the World Cup quarter-finals.
'This is a miracle. Only great teams can be as brave as we were and fight back from a goal down against teams like England'
The comparison with Maradona for the title of the greatest Argentine player of all time cannot be complete without a world title, which the late Maradona won almost single-handedly 36 years ago.
For Croatia coach Zlatko Dalic the final is a chance to settle the score 20 years after the bitter defeat
Croatia's head coach Zlatko Dalic said as he prepares his side for Thursday's decisive Group F showdown.
Croatia midfielder Luka Modric suggested that his team's 2-0 victory over Nigeria on Saturday had turned up the heat on Group D favourites Argentina
Croatia progressed to the semi-finals of the World Cup after a second successive penalty shootout on Saturday but their inability to put away another unimpressive team over 90 minutes and extra time is a major worry for the Balkan side.
They can now dream of going one better than their third-place finish in 1998, which marked their first participation in the World Cup finals as an independent nation.
Croatia had shown glimpses of their potential at the last European Championships in France two years ago when they beat Spain but went out in the second round to eventual winners Portugal after conceding in extra time.
Cristiano Ronaldo, Luka Modric and Mohamed Salah were named as the three finalists.
